|Title||Valve‐Enabled Sample Preparation and RNA Amplification in a Coffee Mug for Zika Virus Detection|
|Publication Type||Journal Article|
|Year of Publication||2018|
|Authors||Jiang, X., J. C. Loeb, C. Manzanas, J. A. Lednicky, and H. Z. Fan|
|Journal||Angew Chem Int Ed Engl|
|Keywords||RNA; detection; isothermal amplification; viruses|
The recent outbreaks of Zika virus (ZIKV) infection represent a public health challenge. Rapid, cost-effective, and reliable diagnostic tools for ZIKV detection at the point of care (POC) are highly desirable, especially for resource-limited nations. To address the need, we have developed an integrated device to achieve sample-to-answer ZIKV detection. The device features innovative ball-based valves enabling the storage and sequential delivery of reagents for virus lysis and a paper-based unit for RNA enrichment and purification. The paper unit is placed in a commercially available coffee mug that provides a constant temperature for reverse transcription loop-mediated isothermal amplification (RT-LAMP), followed by colorimetric detection by naked eye or a cellphone camera. Using the device, we demonstrated the reproducible detection of ZIKV in human urine and saliva samples.