Two-Dimensional Electrophoresis

Sponsored by Army Research Office

Last Modified: Nov 01st 2007

Investigators

Z. Hugh Fan

Student Research Assistants

Champak Das

Description

The project goal is to design and fabricate a microfluidic device that provides an alternative approach to two-dimensional gel electrophoresis, which is extensively used in biological labs. The microfluidics-enabled method likely leads to rapid analysis, high separation resolution, improved protein identification accuracy, and enhanced reproducibility. The device could be used as a tool for biomarker discovery and screening.

Publications

Das, C., Zhang, J., Denslow, N., and Fan, Z.H., "Integration of Isoelectric Focusing with Multi-channel Gel Electrophoresis by Using Microfluidic Pseudo-valves," Lab on a Chip, Vol. 7, pp1806–1812, December 2007. (Abstract)

Das, C., Fredrickson, C.K., Xia, Z., and Fan, Z.H., "Device fabrication and Integration with photodefinable microvalves for protein separation," Sensors and Actuators A: Physical, Vol. 134, No.1, pp.271-277, February 2007.

Das, C., and Fan, Z.H., "Effects of Separation length and voltage on Isoelectric focusing in a plastic microfluidic device," Electrophoresis, 27, 3619-3626, August 2006.

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Two-Dimensional Electrophoresis Sponsored by Army Research Office Last Modified: Nov 01st 2007 Investigators Z. Hugh Fan Student Research Assistants Champak Das Description The project goal is to design and fabricate a microfluidic device that provides an alternative approach to two-dimensional gel electrophoresis, which is extensively used in biological labs. The microfluidics-enabled method likely leads to rapid analysis, high separation resolution, improved protein identification accuracy, and enhanced reproducibility. The device could be used as a tool for biomarker discovery and screening. Publications Das, C., Zhang, J., Denslow, N., and Fan, Z.H., "Integration of Isoelectric Focusing with Multi-channel Gel Electrophoresis by Using Microfluidic Pseudo-valves," Lab on a Chip, Vol. 7, pp1806–1812, December 2007. (Abstract) Das, C., Fredrickson, C.K., Xia, Z., and Fan, Z.H., "Device fabrication and Integration with photodefinable microvalves for protein separation," Sensors and Actuators A: Physical, Vol. 134, No.1, pp.271-277, February 2007. Das, C., and Fan, Z.H., "Effects of Separation length and voltage on Isoelectric focusing in a plastic microfluidic device," Electrophoresis, 27, 3619-3626, August 2006.